首頁 ? LL/2 (LLC1)小鼠肺癌細胞株cell line BioVector NTCC質粒載體菌種細胞基因保藏中心

LL/2 (LLC1)小鼠肺癌細胞株cell line BioVector NTCC質粒載體菌種細胞基因保藏中心

  • 價  格:¥39865
  • 貨  號:LL/2 (LLC1)小鼠肺癌細胞株cell line
  • 產  地:北京
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地址:北京

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LL/2 (LLC1)小鼠肺癌細胞株cell line BioVector NTCC質粒載體菌種細胞基因保藏中心
Organism Mus musculus, mouse
Tissue lung
Product Format frozen
Morphology rounded - loosely attached or floating
Culture Properties mixed, adherent and suspension
Biosafety Level 1
Disease Lewis lung carcinoma
Strain C57BL
Applications This line is widely used as a model for metastasis and is useful for studying the mechanisms of cancer chemotherapeutic agents.
Derivation Lewis lung carcinoma is a cell line established from the lung of a C57BL mouse bearing a tumor resulting from an implantation of primary Lewis lung carcinoma.
Antigen Expression H-2b
Tumorigenic Yes
Effects Yes, in C57BL mice
Comments The cells are resistant to 1,3-bis-(2-chloroethyl)-1-nitrosourea, but are sensitive to methotrexate.
The cells are reported to be highly tumorigenic, but weakly metastatic in mice.
The cells form multilayers in flasks without actually becoming confluent.
Tested and found negative for ectromelia virus (mousepox).
Image

Complete Growth Medium The base medium for this cell line is Dulbecco's Modified Eagle's Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Subcultures are prepared by diluting the suspension 1:4 to 1:6. Cells on the floor of the flask may be dislodged by aspirating several times with culture medium or by rinsing with 0.25% trypsin - 0.53 mM EDTA solution. Corning? T-75 flasks (catalog #430641) are recommended for subculturing this product.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions Temperature: 37°C

【Supplier來源】BioVector NTCC Inc.
TEL:+86-010-53513060
【Website網址】 http://www.biovector.net

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