首頁 ? pET His10 MBP Asn10 TEV LIC cloning vector (2CT-10) plasmid vector質粒載體 BioVector NTCC質粒載體菌種細胞基因保藏中心

pET His10 MBP Asn10 TEV LIC cloning vector (2CT-10) plasmid vector質粒載體 BioVector NTCC質粒載體菌種細胞基因保藏中心

  • 價  格:¥19865
  • 貨  號:pET His10 MBP Asn10 TEV LIC cloning vector (2CT-10) plasmid vector質粒載體
  • 產  地:北京
點擊詢問我要采購
 竭誠為您服務!
BioVector NTCC典型培養物保藏中心
聯系人:Dr.Xu, Biovector NTCC Inc.點擊這里給我發消息

電話:+86-010-53513060. 工作QQ:1843439339

郵件:[email protected]

手機:18901268599

地址:北京

已注冊
 
pET His10 MBP Asn10 TEV LIC cloning vector (2CT-10) plasmid vector質粒載體 BioVector NTCC質粒載體菌種細胞基因保藏中心
This plasmid is an empty vector. Your gene can be inserted with a LIC cloning protocol. All 2-series vectors work as single-expression vectors, as well as transfer vectors for our polycistronic system.

The LIC cloning site is flanked by 5 pairs of restriction sites, so that your gene can easily be subcloned into our polycistronic destination vectors (2D, 2E, or 2Z).

2CT-10 has a TEV-cleavable N-terminal His10-MBP fusion tag followed by Asn10. MBP can improve the expression and solubility of your target protein. The N10 linker may help you to avoid steric clashes between your protein and the MBP tag.

To clone into this vector, add LIC v1 tags to the 5' end of your PCR primers.

Forward - 5'-TACTTCCAATCCAATGCA-3'
Reverse - 5'-TTATCCACTTCCAATGTTATTA-3'

Linearize the plasmid with SspI and gel purify.

When digesting the DNA with T4 polymerase, use dCTP for insert and dGTP for vector.
【Supplier來源】BioVector NTCC Inc.
TEL:+86-010-53513060
【Website網址】 http://www.biovector.net

您正在向 biovector.net  發送關于產品 pET His10 MBP Asn10 TEV LIC cloning vector (2CT-10) plasmid vector質粒載體 BioVector NTCC質粒載體菌種細胞基因保藏中心 的詢問

點擊“立即發送”后,我們將在1個工作日內與您取得聯系。

 

浙江体彩6+1玩法 中彩神游戏 大发娱乐首页 投资2015小本赚钱加盟 东北人靠小女孩赚钱 趣头条能赚钱的app下载 给孩子玩的地方赚钱 未来10最赚钱的行业6 516威趣游戏 金蟾捕鱼 蛋咖赚钱好用吗 微乐游戏南昌麻将 龙王捕鱼必赢窍门 各大赚钱故事 河北麻将微信群 开少儿英语班赚钱么 缅甸当兵赚钱吗 qq麻将怎么开好友房