首頁 ? MC38-OVA cell line細胞株細胞系MC-38-OVA BioVector NTCC質粒載體菌種細胞基因保藏中心

MC38-OVA cell line細胞株細胞系MC-38-OVA BioVector NTCC質粒載體菌種細胞基因保藏中心

  • 價  格:¥989865
  • 貨  號:MC38-OVA cell line細胞株細胞系MC-38-OVA
  • 產  地:北京
點擊詢問我要采購
 竭誠為您服務!
BioVector NTCC典型培養物保藏中心
聯系人:Dr.Xu, Biovector NTCC Inc.點擊這里給我發消息

電話:+86-010-53513060. 工作QQ:1843439339

郵件:[email protected]

手機:18901268599

地址:北京

已注冊
 

MC38-OVA cell line細胞株細胞系MC-38-OVA BioVector NTCC質粒載體菌種細胞基因保藏中心


The colon carcinoma cell line MC38 expressing OVA was generated by transduction of MC38 cells with LZRS-based OVA-IRES-EGFP retrovirus. Tumor cells were maintained in IMDM containing 9% heat-inactivated FCS, 2 mM glutamine, 2-ME, penicillin, and streptomycin.

Organism: Mus musculus (mouse) , C57BL6 murine
Organ: colon
Disease: colorectal adenocarcinoma
Cell Type: epithelial-like
Growth Properties: adherent
Medium: IMDM containing 9% heat-inactivated FCS, 2 mM glutamine, 2-ME, penicillin, and streptomycin..
Tumorigenic: Yes
Subculturing Protocol:
1. Remove and discard culture medium.
2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
5. Add appropriate aliquots of the cell suspension to new culture vessels.
6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:5 to 1:10 is recommended
Medium Renewal: 2 to 3 times per week
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase

【Supplier來源】BioVector NTCC Inc.
TEL:+86-010-53513060
【Website網址】 http://www.biovector.net

您正在向 biovector.net  發送關于產品 MC38-OVA cell line細胞株細胞系MC-38-OVA BioVector NTCC質粒載體菌種細胞基因保藏中心 的詢問

點擊“立即發送”后,我們將在1個工作日內與您取得聯系。

 

浙江体彩6+1玩法 北京快乐8中奖规则 河南泳坛夺金基本走势 河南体彩泳坛夺金今天 三国麻将风云在线玩 白小姐精选四不像大全 大发快三赢钱投注 广东36选7复式计算器 股票分析方法和逻辑 重庆百变王牌前三走势图 广东好彩1最新开奖结果查询 资料2019 富贵乐园游戏 有秒秒彩的彩票平台 启运配资 宁夏11选5在线购买 体彩湖南幸运赛车